Mavlyanova Shakhnoza Zakirovna
The aim of the study was to study the variability of genomic complexes of staphylococci isolated from the skin in patients with allergic dermatoses. Material and research methods. Molecular genetic studies were carried out in 63 cultures of st.aureus isolated from the skin of lesions in patients with allergic dermatoses. The material for DNA was a scraping of microbial cell cultures from a Petri dish. DNA was isolated from the samples using the RIBO-prep reagent kit (manufactured by InterLabService LLC, Moscow, Russia). The results of the study. The results of molecular genetic studies of st.aureus genotypes showed that of the presented samples, methicillin-resistant staphylococcus - (mecA) was found in 55 patients, which amounted to 87.3% of cases. Whereas staphylococci producing toxic shock syndrome toxin - (Tsst) were found in 30 patients (47.6%), and staphylococci producing Panton-Walentine leukocidin - Luk - 433 bp were found in 7, which amounted to 11.1%, respectively . Combined genotypes of methicillin-resistant staphylococcus (mecA) and toxic shock syndrome toxin (Tsst) were observed, which were detected in 29 patients, which accounted for 46.03% of cases and 7 patients noted a combination of MRSA + Tsst + Luk - 433 bp, which was 11, 1% of cases.An analysis of the clinical course of allergic dermatoses taking into account the isolated strains of S. Aureus showed that in the group of patients with blood pressure with MRSA, the clinical picture of the skin-pathological process was characteristic of erythematic-squamous character with lichenification. In patients with a TSST strain of S. Aureus, the skin-pathological process was widespread, infiltrative, caused by erythematous, vesicular rashes, and large-lamellar peeling in the form of scalded skin on the upper and lower extremities was noted.In patients with the genotype, the pore-forming toxin Panton-Valentine leukocidin (PVL), the skin-pathological process had a common, infiltrative-inflammatory nature, with a disease duration of more than 10 years with recurrent relapses and resistance to the standard therapy.